RIBOSOME PRECURSOR PARTICLES IN NUCLEOLI
نویسندگان
چکیده
منابع مشابه
Ribosome Precursor Particles in Nucleoli
Ribonucleoprotein (RNP) particles containing the precursors of ribosomal RNA were extracted from L cell nucleoli and analyzed under conditions comparable to those used in the characterization of cytoplasmic ribosomes. Using nucleoli from cells suitably labeled with (3)H-uridine, we detected three basic RNP components, sedimenting at approximately 62S, 78S, and 110S in sucrose gradients containi...
متن کاملUltrastructural and Biochemical Studies on the Precursor Ribosomal Particles Isolated from Rat Liver Nucleoli
Sucrose density gradient analyses of pH 5.5 and pH 7.4 extracts from rat liver nucleoli revealed the presence of two broad peaks of approximately 60S and 80S, and 60S and 80-100S, respectively. Ribonucleoprotein (RNP) particles containing precursor ribosomal RNA in these peaks have been characterized by electron microscopy and RNA analyses. Spherical particles only were found in the 60S peak of...
متن کاملA serum albumin precursor in cytoplasmic particles.
The existence of an intermediate in the biosynthesis of serum albumin was suggested by a previous study of the kinetics of appearance of Cl4 in soluble serum albumin in liver slices (1). In the course of investigations on the nature of this intermediate, it has been found that treatment of cytoplasmic particles of chicken liver with deoxycholate or other agents will release non-dialyzable mater...
متن کاملIsolation of ribosome particles from meningopneumonitis organisms.
In ribonucleic acid (RNA) extracted by phenol and sodium dodecyl sulfate from purified reticulate bodies of meningopneumonitis (MP) organisms, 21S, 16S, and 4S RNA were found by sucrose density gradient sedimentation analysis. When purified reticulate bodies were homogenized by sonic treatment or by treatment with sodium deoxycholate and were fractionated by differential centrifugation, more th...
متن کاملFormation of Ribosomes from Precursor Ribonucleoprotein Particles.
Sypherd, Paul S. (University of California, San Diego). Formation of ribosomes from precursor ribonucleoprotein particles. J. Bacteriol. 90:411-417. 1965.-Amino acid deprivation of a "relaxed" mutant leads to the accumulation of ribonucleoprotein particles. The possibility that these particles are equivalent to normal ribosome precursors was investigated. This was accomplished by determining th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1969
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.42.1.272